| Group | Team | P | W | D | L | GD | PTS |
|---|---|---|---|---|---|---|---|
| MLS NEXT Pro |  Chattanooga FC |
10 | 7 | 2 | 1 | 7 | 23 |
| MLS NEXT Pro |  Ventura County FC |
11 | 7 | 0 | 4 | 4 | 21 |
| MLS NEXT Pro |  St. Louis City 2 |
11 | 6 | 1 | 4 | 2 | 19 |
| MLS NEXT Pro |  New York RB II |
9 | 5 | 2 | 2 | 3 | 17 |
| MLS NEXT Pro |  Colorado Rapids 2 |
10 | 5 | 2 | 3 | 2 | 17 |
| MLS NEXT Pro |  North Texas SC |
10 | 5 | 2 | 3 | -2 | 17 |
| MLS NEXT Pro |  Huntsville City FC |
10 | 4 | 4 | 2 | 11 | 16 |
| MLS NEXT Pro |  Houston Dynamo 2 |
10 | 5 | 1 | 4 | 4 | 16 |
| MLS NEXT Pro |  Portland Timbers 2 |
11 | 5 | 1 | 5 | -1 | 16 |
| MLS NEXT Pro |  Tacoma Defiance |
9 | 5 | 0 | 4 | 3 | 15 |
| MLS NEXT Pro |  New York City FC II |
12 | 4 | 3 | 5 | -3 | 15 |
| MLS NEXT Pro |  Los Angeles FC II |
10 | 4 | 2 | 4 | -2 | 14 |
| MLS NEXT Pro |  Philadelphia Union II |
7 | 3 | 4 | 0 | 10 | 13 |
| MLS NEXT Pro |  Minnesota United 2 |
7 | 4 | 1 | 2 | 5 | 13 |
| MLS NEXT Pro |  Chicago Fire FC II |
9 | 4 | 1 | 4 | -4 | 13 |
| MLS NEXT Pro |  Orlando City B |
9 | 4 | 1 | 4 | -4 | 13 |
| MLS NEXT Pro |  New England Revolution II |
8 | 3 | 3 | 2 | 3 | 12 |
| MLS NEXT Pro |  Toronto FC II |
10 | 3 | 2 | 5 | -6 | 11 |
| MLS NEXT Pro |  The Town FC |
8 | 3 | 1 | 4 | 4 | 10 |
| MLS NEXT Pro |  Crown Legacy FC |
8 | 2 | 4 | 2 | 1 | 10 |
| MLS NEXT Pro |  Austin FC II |
9 | 2 | 4 | 3 | -3 | 10 |
| MLS NEXT Pro |  Inter Miami CF II |
10 | 3 | 1 | 6 | -6 | 10 |
| MLS NEXT Pro |  Vancouver Whitecaps 2 |
11 | 2 | 3 | 6 | 0 | 9 |
| MLS NEXT Pro |  Real Monarchs |
7 | 2 | 3 | 2 | 0 | 9 |
| MLS NEXT Pro |  Atlanta United 2 |
9 | 1 | 6 | 2 | -4 | 9 |
| MLS NEXT Pro |  Carolina Core |
9 | 1 | 5 | 3 | 0 | 8 |
| MLS NEXT Pro |  Columbus Crew 2 |
8 | 2 | 2 | 4 | -4 | 8 |
| MLS NEXT Pro |  FC Cincinnati 2 |
8 | 1 | 2 | 5 | -4 | 5 |
| MLS NEXT Pro |  Sporting Kansas City II |
10 | 0 | 3 | 7 | -16 | 3 |
- P: Played
- W: Wins
- D: Draws
- L: Losses
- GD: Goal Difference
- PTS: Points
- Default
- Green
- Blue
- Pink
- Yellow
- Orange
- Purple
- Red
- Lightblue
- Teal
- Lime
- Deeporange